The mass spectrometer directly monitors the biological fermentation tail gas method

In 2010, Shanghai Hanyu Hengping Scientific Instrument Co., Ltd. undertook the Shanghai Municipal Science and Technology Commission's "Method for Direct Analysis of Biofermented Exhaust Gases by Domestic Quadrupole Mass Spectrometers" project. After a year of research, the project passed the Shanghai Branch a few days ago. Commission’s acceptance by the expert group. Professor Zhang Xiaoliang of the National Biochemical Engineering Technology Research Center of East China University of Technology serves as the head of the expert group. The expert group is composed of experts from different fields such as government inspection agencies and universities.

Biofermentation involves many fields such as medicine, light industry, food, agriculture, oceanography, and environmental protection. It plays an extremely important role in the development of China's national economy. It is a technological field that is currently in urgent need of economic and social development, and it is also a hot area for development in countries around the world. . In the process of biological fermentation, the detection of various gas components in the fermentation tail gas has a very important position. Changes in the composition of the fermentation tail gas reflect the changes in the material throughout the fermentation process. Analysis of tail gas data can play a role in monitoring the fermentation process.

During the completion of the project, the project team optimized the exhaust gas pretreatment, collection, analysis, and data processing according to the characteristics of the fermentation tail gas and the requirements of the field application environment, and finally established a set of “online mass spectrometer direct”. "Methods for Analysis of Bioferment Exhaust Gases" and standard operating procedures. The SHP8400PMS online mass spectrometer can directly analyze the fermentation tail gas and realize real-time automatic on-line monitoring. It can obtain continuous and stable accurate measurement results, and perform high-precision quantitative analysis on oxygen, carbon dioxide, nitrogen, argon and various volatile substances. Increased monitoring efficiency. At present, this method has been successfully applied in the fermentation engineering research of the National Biochemical Engineering Technology Research Center (Shanghai) and the production site monitoring of a number of biopharmaceutical companies. It has the exemplary significance of popularization and application, and lays the foundation for the establishment of industry standard methods. While granting the project a positive and highly appraised opinion, the expert group also put forward fairly relevant research proposals, hoping to better apply the domestic mass spectrometer to field monitoring.